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Identification of superior lipid producing lipomyces and myxozyma yeasts
- Bruce S. Dien, Patricia J. Slininger, Cletus P. Kurtzman, Bryan R. Moser, Patricia J. O'Bryan
- AIMS environmental science 2016 v.3 no.1 pp. 1-20
- Lipomyces, Rhodosporidium toruloides, biomass, environmental science, fuels, glucose, hydrolysates, lipids, oils, xylose, yeasts
- Oleaginous yeasts are of interest for production of single cell oils from sugars. Eighteen members of the Lipomyces and Myxozyma clade were screened for lipid production when cultured on 10%w/v glucose. The highest ranking yeasts included L. tetrasporus (21 g/L), L. spencer-martinsiae (19.6 g/L), and L. lipofer (16.7 g/L). By contrast, Rhodosporidium toruloides, which was included as a positive control, produced 16.7 g/L. The L. tetrasporus and L. lipofer were further characterized for growth and lipid production on sugars present in biomass hydrolysates. These included L-arabinose, xylose, and an equal glucose and xylose mixture. L. tetrasporus had lipid titers of 16.3–20.8 g/L and L. lipofer 12.5–17.0 g/L. When both strains were grown on an equal mixture of glucose and xylose, xylose was consumed immediately following glucose. Lipid contents for the yeasts consisted primarily of C18:1 and C16:0, which makes them a promising source of lipids for fuel applications.