Main content area

An esterase AppH for the hydrolysis of 2-(4-aryloxyphenoxy) propionate herbicides in Sphingobium sp. strain C3

Chen, Dian, Kong, Xiangkun, Wu, Shurui, Huang, Junwei, Cheng, Xiaokun, Jiang, Jiandong
International biodeterioration & biodegradation 2019 v.136 pp. 34-40
Sphingomonas, catalytic activity, esterases, esters, fenoxaprop, genes, hydrolysis, polluted soils, propionic acid, weeds
AOPP herbicides are widely used against monocotyledonous weeds in agriculture. In this study, the Sphingobium sp. strain C3 was isolated from an AOPP herbicide-contaminated soil and was able to transform various AOPP herbicides, including the most commonly used AOPP herbicide, Fenoxaprop-p-ethyl (FE). The appH gene involved in the transformation of FE to fenoxaprop acid (FA) was cloned from strain C3. AppH showed 23–36% identity with other α/β-hydrolases and belongs to subfamily Ⅵ of bacterial esterases. The recombinant AppH, which was highly expressed and purified, was able to transform many AOPP herbicides and p-nitrophenyl esters. AppH showed the highest catalytic activity towards Clodinafop-propargyl among the AOPP herbicides tested, and the catalytic activity decreased with the increase in the aliphatic chain length of AOPP herbicides. This study provides a new bacterial strain and a novel esterase for the initial hydrolysis of AOPP herbicides.