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An esterase AppH for the hydrolysis of 2-(4-aryloxyphenoxy) propionate herbicides in Sphingobium sp. strain C3

Author:
Chen, Dian, Kong, Xiangkun, Wu, Shurui, Huang, Junwei, Cheng, Xiaokun, Jiang, Jiandong
Source:
International biodeterioration & biodegradation 2019 v.136 pp. 34-40
ISSN:
0964-8305
Subject:
Sphingomonas, catalytic activity, esterases, esters, fenoxaprop, genes, hydrolysis, polluted soils, propionic acid, weeds
Abstract:
AOPP herbicides are widely used against monocotyledonous weeds in agriculture. In this study, the Sphingobium sp. strain C3 was isolated from an AOPP herbicide-contaminated soil and was able to transform various AOPP herbicides, including the most commonly used AOPP herbicide, Fenoxaprop-p-ethyl (FE). The appH gene involved in the transformation of FE to fenoxaprop acid (FA) was cloned from strain C3. AppH showed 23–36% identity with other α/β-hydrolases and belongs to subfamily Ⅵ of bacterial esterases. The recombinant AppH, which was highly expressed and purified, was able to transform many AOPP herbicides and p-nitrophenyl esters. AppH showed the highest catalytic activity towards Clodinafop-propargyl among the AOPP herbicides tested, and the catalytic activity decreased with the increase in the aliphatic chain length of AOPP herbicides. This study provides a new bacterial strain and a novel esterase for the initial hydrolysis of AOPP herbicides.
Agid:
6252140