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Degradation of scrap tyre by Bacillus sp. – Kinetic aspects of major environmental parameters and identification of potential growth substrates

Author:
Lao, Weng Chon, Alves de Toledo, Renata, Lu, Qihong, Shim, Hojae
Source:
International biodeterioration & biodegradation 2019 v.137 pp. 95-101
ISSN:
0964-8305
Subject:
Bacillus (bacteria), alkanes, dissolved oxygen, environmental factors, gas chromatography-mass spectrometry, inoculum, pH, phenol, specific growth rate, surface area, tires, wastes
Abstract:
This study aimed to elucidate some kinetic aspects involved during the degradation of waste scrap tyre by a Bacillus sp. previously isolated. After a 5-day cultivation, 2.03 ± 0.22% and 0.005 ± 0.001% of weight loss were observed for tyre sample and control group, respectively. The potential organic compounds leached out from tyre, identified by GC-MS, mainly consisted of n-alkanes and some alkylated phenols. A mixture of tetradecane, nonadecane, and 2,4-di-tert-butylphenol (60 mg l−1 total concentration) supported the isolate growth during the 5-day cultivation. The n-alkanes were removed more effectively (tetradecane, 64.93% and nonadecane, 47.92%) than the alkylated phenol (3.03%), contributing to the tyre degradation by Bacillus sp. observed. The optimal conditions were determined as 6.70 mg l−1 dissolved oxygen concentration, pH 7, 10% (v/v) inoculum size, 30 °C, and 120 mm2 tyre surface area. The highest maximum specific growth rate (μmax) (0.06 OD unit d−1 for S and SI) and the shortest lag phases (0.28 d for S and 0.17 d for SI) were obtained under the optimized conditions for the Bacillus sp. incubation. The electron micrographs showed the isolate ability to colonize the waste tyre surface.