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Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

Nunes, Márcio Roberto Teixeira, de Souza, William Marciel, Savji, Nazir, Figueiredo, Mário Luís, Cardoso, Jedson Ferreira, da Silva, Sandro Patroca, da Silva de Lima, Clayton Pereira, Vasconcelos, Helena Baldez, Rodrigues, Sueli Guerreiro, Ian Lipkin, W., Vasconcelos, Pedro Fernando Costa, Palacios, Gustavo
Infection, genetics, and evolution 2019 v.68 pp. 16-22
Oropouche orthobunyavirus, arboviruses, disease outbreaks, genome, genotype, molecular epidemiology, public health, reverse transcriptase polymerase chain reaction, Amazonia, Brazil
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.