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An efficient nucleic acids extraction protocol for Elettaria cardamomum

Author:
Palani, Sankara Naynar, Elangovan, Sangeetha, Menon, Aathira, Kumariah, Manoharan, Tennyson, Jebasingh
Source:
Biocatalysis and agricultural biotechnology 2019 v.17 pp. 207-212
ISSN:
1878-8181
Subject:
DNA, Elettaria cardamomum, ascorbic acid, cardamom, cetyltrimethylammonium bromide, cryopreservation, gene expression, genes, genetic markers, genomics, messenger RNA, polyphenols, polysaccharides, random amplified polymorphic DNA technique, reverse transcriptase polymerase chain reaction, sodium dodecyl sulfate
Abstract:
Elettaria cardamomum is an economically important spice crop. Genomic analysis of cardamom is often faced with the limitation of inefficient nucleic acid extraction due to its high content of polyphenols and polysaccharides. In this study, a highly efficient DNA and RNA extraction protocol for cryopreserved samples from small cardamom plant was developed with modification in the CTAB and SDS method for DNA and RNA, respectively, with the inclusion of 2% ascorbic acid. DNA isolated by this method is highly suitable for PCR, restriction digestion and RAPD analysis. The RNA extraction method described here represent the presence of plant mRNA, small RNAs and viral RNA and, the isolated RNA proved amenable for RT-PCR and amplification of small and viral RNA. Nucleic acids extraction protocol developed here will be useful to develop genetic marker for cardamom, to clone cardamom genes, small RNAs and cardamom infecting viral genes and to perform gene expression and small RNA analysis.
Agid:
6252854