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Determination of squalene in edible oils by transmethylation and GC analysis
- Budge, Suzanne M., Barry, Christopher
- MethodsX 2019 v.6 pp. 15-21
- catalysts, cooking fats and oils, flame ionization, functional foods, reference standards, squalene, therapeutics, triterpenoids
- Squalene is a triterpenoid with medicinal, therapeutic and cosmetic applications. There is interest in identifying novel sources of squalene, creating a need for a rapid and accurate method of squalene determination. Here, we describe such an approach that involves first transmethylating the oil base containing squalene using a basic catalyst, followed by addition of internal standard and quantification by gas chromatography flame ionization detection (GCIFD).The method uses a single reference standard for quantification. Validation showed it to be both repeatable and accurate. It was successfully applied to nutraceutical and edible oils containing squalene.