PubAg

Main content area

Dietary flavonoids from Allium mongolicum Regel promotes growth, improves immune, antioxidant status, immune-related signaling molecules and disease resistance in juvenile northern snakehead fish (Channa argus)

Author:
Li, Muyang, Zhu, Xinming, Tian, Jiaxin, Liu, Ming, Wang, Guiqin
Source:
Aquaculture 2019 v.501 pp. 473-481
ISSN:
0044-8486
Subject:
Aeromonas hydrophila, Allium, Channa argus, alanine transaminase, antioxidants, aspartate transaminase, blood serum, catalase, cortisol, disease resistance, fish, flavonoids, food intake, gene expression regulation, glutathione peroxidase, immune response, immunoglobulin M, interleukin-1beta, interleukin-8, juveniles, kidneys, liver, lysozyme, specific growth rate, superoxide dismutase, survival rate, transcription factor NF-kappa B, tumor necrosis factor-alpha, weight gain
Abstract:
The present study was conducted to evaluate the effects of dietary flavonoids from Allium mongolicum Regel (AMRF) supplementation on growth, antioxidant and immune status, immune-related genes expression and disease resistance in Channa argus. A basal diet was supplemented with AMRF at 0, 10, 20 or 40 mg/kg feed for 56 days. The result revealed that weight gain (WG) and specific growth rate (SGR) were significantly increased (P < .05) with the increase of dietary AMRF levels from 0 to 40 mg/kg. All AMRF-supplemented groups (10, 20 or 40 mg/kg) significantly enhanced (P < .05) serum AKP, lysozyme activity, C3, IgM concentration, liver antioxidant enzymes activity (SOD, CAT and GSH-Px) or decreased (P < .05) serum cortisol, liver MDA content, serum ALT and AST activity. Meanwhile, AMRF administration significantly up-regulated (P < .05) the expression of SOD, IgM, HSP70, HSP90, IκB-α and GR or down-regulated (P < .05) NF-κB p65 and inflammatory cytokines (TNF-α, IL-1β and IL-8) transcription in head kidney of C. argus. After challenge with Aeromonas hydrophila, survival rates in all AMRF treatment groups were significantly increased (P < .05). These results indicated that dietary intake containing AMRF enhanced growth, antioxidant and immune response and regulated immune-related genes expression and increased disease resistance against Aeromonas hydrophila in snakehead fish, with greatest effects in fish given 40 mg/kg AMRF for 56 days.
Agid:
6253519