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Characterization of polysaccharides isolated from Periploca angustifolia and its antioxidant activity and renoprotective potential against cadmium induced toxicity in HEK293 cells and rat kidney

Athmouni, Khaled, Belhaj, Dalel, Chawech, Rachid, Jarraya, Raoudha, El Feki, Abdelfattah, Ayadi, Habib
International journal of biological macromolecules 2019 v.125 pp. 730-742
antioxidant activity, blood serum, cadmium, catalase, creatinine, glycation, high performance liquid chromatography, histopathology, kidneys, lipid peroxidation, oxidation, oxidative stress, polysaccharides, rats, superoxide dismutase, toxicity, urea
Periploca angustifolia has numerous biological properties and also used against various diseases. In our study, this plant has been used for biotechnological production of polysaccharides. The prevention action of polysaccharides isolated from P. angustifolia (PAPS) against Cd-caused oxidative stress in HEK293 cells and kidneys of rats was tested. PAPS were characterized by HPLC, FT-IT, DRX, 1D-and 2D-MNR. PAPS present strong capacity to slow the rate of lipid peroxidation and protein glycation in vitro. In addition, the results indicated that the Cd treatment caused a significant decrease in HEK293 cells viability which wasattenuated by PAPS pre-treatment. Furthermore, our findings revealed that Cd injection increased the levels of urea and creatinine in the serum. The increased levels of protein oxidation and lipid peroxidation along with decreased activities of SOD, CAT and GPx were significantly (p < 0.01) ameliorated by PAPS pre-treatment. Finally, histopathological studies also supported the prevention action of PAPS.