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Analysis of interaction between sulfated polysaccharides and HIV oligopeptides by surface plasmon resonance

Author:
Battulga, Tungalag, Tumurbaatar, Oyunjargal, Ganzorig, Oyundelger, Ishimura, Takahisa, Kanamoto, Taisei, Nakashima, Hideki, Miyazaki, Kensuke, Yoshida, Takashi
Source:
International journal of biological macromolecules 2019 v.125 pp. 909-914
ISSN:
0141-8130
Subject:
Human immunodeficiency virus, antiretroviral agents, antiretroviral properties, arginine, curdlan, dextran, dissociation, electrostatic interactions, glycoproteins, light scattering, lysine, models, molecular weight, oligopeptides, particle size, sulfates, surface plasmon resonance, zeta potential
Abstract:
This study aims to quantitatively investigate the interaction between sulfated polysaccharides with potent anti-HIV activity, dextran and curdlan sulfates with negatively charged sulfate groups, and poly-L-lysine as a model protein and oligopeptides from a HIV surface glycoprotein gp120 with positively charged amino acids using surface plasmon resonance (SPR) and dynamic light scattering (DLS) to elucidate the anti-HIV mechanism of sulfated polysaccharides. The apparent association- (ka) and dissociation rate (kd) constants of dextran and curdlan sulfates against poly-L-lysine were ka = 6.92 × 104–2.17 × 106 1/Ms and kd = 4.29 × 10−5–2.22 × 10−4 1/s; these kinetic constants were dependent on the molecular weights and degree of sulfation of sulfated polysaccharides. For interaction, the three oligopeptides from the HIV gp120 were peptide A 297TRPNNNTRKRIRIQRGPGRA316 with several lysine (K) and arginine (R) in the V3 loop region, peptide B 493PLGVAPTKAKRRVVQREKR511 with several K and R in the C-terminus region, and oligopeptide C 362KQSSGGDPEIVTHSFNCGG380 with few basic amino acids in the CD4 binding domain. Sulfated polysaccharides exhibited strong interaction against oligopeptides A and B, (ka = 5.48 × 104–2.96 × 106 1/Ms. and kd = 1.74 × 10−4–6.24 × 10−3 1/s), no interaction was noted against oligopeptide C. Moreover, the particle size and zeta potential by DLS indicated the interaction between sulfated polysaccharides and oligopeptides A and B, suggesting the anti-HIV mechanism of sulfated polysaccharides to be the electrostatic interaction of negatively charged sulfated polysaccharides and HIV at the positively charged amino acid regions.
Agid:
6254015