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A thermosensitive RGD-modified hydroxybutyl chitosan hydrogel as a 3D scaffold for BMSCs culture on keloid treatment

Qu, Congcong, Bao, Zixian, Zhang, Xin, Wang, Zhiguo, Ren, Jizhen, Zhou, Zhongzheng, Tian, Meiping, Cheng, Xiaojie, Chen, Xiguang, Feng, Chao
International journal of biological macromolecules 2019 v.125 pp. 78-86
Fourier transform infrared spectroscopy, adhesion, biocompatibility, cell culture, chitosan, fibroblasts, hydrogels, mesenchymal stromal cells, nuclear magnetic resonance spectroscopy, therapeutics, thermosensitivity
Cell therapy with bone marrow-derived mesenchymal stem cells (BMSCs) is a potential method for many disease treatments, including keloid. In the present study, an Arg-Gly-Asp (RGD) modified hydroxybutyl chitosan (HBC) hydrogel (HBC-RGD) was developed to enhance the adhesion and proliferation of BMSCs within the hydrogel. The successful synthesis of HBC-RGD was confirmed by FTIR and 1H NMR. Both HBC and HBC-RGD hydrogel had desired thermosensitivity, biocompatibility and enzymatic degradability in vitro. Compared with HBC hydrogel, HBC-RGD hydrogel was more beneficial for the adhesion and proliferation of BMSCs. Furthermore, the BMSCs incorporated HBC-RGD (BMSCs/HBC-RGD) hydrogel could inhibit the proliferation of keloid fibroblasts (Kfs) and suppress the nodular collagenous fibers of keloid tissue. These results suggested that the HBC-RGD hydrogel could be applied as a potential 3D hydrogel scaffold for cell culture, and BMSCs/HBC-RGD hydrogel was potential to be applied for keloid therapy with subcutaneous in-situ injection in the future.