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Development of Potato virus Y (PVY) resistant pepper (Capsicum annuum L.) lines using marker-assisted selection (MAS)
- Moodley, Vaneson, Naidoo, Roobavathie, Gubba, Augustine, Mafongoya, Paramu L.
- Physiological and molecular plant pathology 2019 v.105 pp. 96-101
- Capsicum annuum, Potato virus Y, alleles, crop production, genotype, germplasm, loci, marker-assisted selection, pepper, polymerase chain reaction, recessive genes
- Several alleles at the pvr2 locus are known to control recessive resistance to Potato virus Y (PVY) in pepper. In this study, PVY resistant F2 pepper lines were developed from local germplasm carrying recessive genes (pvr21 and pvr22) using marker-assisted selection (MAS). The F1 and F2 generations were assessed for the presence of resistant/susceptible alleles (pvr2+/pvr21/pvr22) at the pvr2-elF4E locus using the tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) procedure. Our results show that ARMS-PCR can be used to successfully screen pepper genotypes for alleles that confer PVY resistance thereby contributing to the improvement of pepper production.