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Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

Author:
Taguchi, Shogo, Suga, Keishi, Hayashi, Keita, Yoshimoto, Makoto, Okamoto, Yukihiro, Nakamura, Hidemi, Umakoshi, Hiroshi
Source:
Colloids and surfaces 2019 v.175 pp. 403-408
ISSN:
0927-7765
Subject:
chlorophyll, colloids, lipid content, micelles, mixing ratio, phospholipids
Abstract:
The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.
Agid:
6255949