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Physicochemical and microbiological monitoring of anaerobic digestion at laboratory and low cost tubular digester

Quintero, M., Alzate, M., Patiño, F., Arboleda, A., Pinzón, Y., Mantilla, A., Escalante, H.
Bioresource technology 2019 v.274 pp. 321-328
Archaea, anaerobic digestion, bacteria, biogas, cattle manure, chemical oxygen demand, cost effectiveness, methane, methane production, mixing ratio, monitoring, most probable number technique, pH, rural areas, volatile fatty acids
The aim of this work was to use an inexpensive technique, such as the most probable number, MPN to quantitatively and qualitatively examine microbial groups in anaerobic digestion, AD of cattle manure, CM. Different diluents and mixing ratios were evaluated in 100 mL reactors using biomethane potential, BMP, as output variable. MPN tests were performed to samples from a 23 L tank and 6.5 m3 low-cost tubular digester, LCTD. To monitor LCTD stability, volatile solids, VS, specific methanogenic activity, SMA, pH, volatile fatty acids, VFA, and chemical oxygen demand, COD were measured. Microbial characterization highlighted the optimal population essential to initiate the anaerobic digestion process. Bacteria domain were predominant over Archaea, with average abundance values of 78% and 22%, respectively. SMA of 0.0486 g COD g−1 SV d−1 was obtained at laboratory-scale and LCTD reached 83% of this value. Conditions selected are cost-effective to establish a biodigester in rural areas.