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Proteomic profiles of self-incompatible and self-compatible pollen-pistil interactions in almond by iTRAQ and 2D-nano-LC ESI-MSMS

Author:
Gómez, E. M., Dicenta, F., Ortega, E.
Source:
Acta horticulturae 2018 no.1219 pp. 207-212
ISSN:
0567-7572
Subject:
Prunus dulcis, almonds, gametophytes, gene expression regulation, genes, loci, phenotype, pistil, pollen, pollination, protein composition, proteins, proteomics, ribonucleases, stress tolerance
Abstract:
The cultivated almond [Prunus dulcis (Miller) D.A. Webb] shows gametophytic self-incompatibility regulated by the S locus. This locus contains two highly linked genes that codify for the S-RNase (pistil determinant) and the SFB protein (pollen determinant), which determine the specificity of incompatibility. However, there are studies that support the involvement of other modifier components in the incompatibility system of this species. A few proteomic studies have tried to identify candidates to modifier factors of the incompatibility system in Prunus sp., using comparative proteomics of pollinated pistils. However, there are no studies in which self-incompatible and self-compatible pollen-pistil interactions are compared. To identify differentially expressed protein profiles in almonds with the Sf-haplotype but different incompatibility phenotype, iTRAQ and 2D-nano-LC ESI-MSMS were carried out. Eight hundred and ninety five proteins were identified as differentially expressed. Most of these proteins had a metabolic or stress resistance and defence function in all the conditions and the up-regulation was higher after pollination. These results provide proteomic profiles of (in)compatibility pollen-pistil interactions, and could also be useful as reference for other comparative proteomic studies of the gametophytic incompatibility.
Agid:
6256380