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Separation and purification of steroidal saponins from Paris polyphylla by microwave‐assisted extraction coupled with countercurrent chromatography using evaporative light scattering detection
- Xiao, Xiao‐Hua, Yuan, Zhi‐Quan, Li, Gong‐Ke
- Journal of separation science 2014 v.37 no.6 pp. 635-641
- countercurrent chromatography, high performance liquid chromatography, light scattering, nuclear magnetic resonance spectroscopy, steroid saponins
- A method of microwave‐assisted extraction coupled with countercurrent chromatography using evaporative light scattering detection was successfully developed for the separation and purification of steroidal saponins from Paris polyphylla. The main extraction conditions including microwave power, liquid/solid ratio, irradiation time, and extraction temperature were optimized using an orthogonal array design method. A suitable two‐phase solvent system consisting of n‐heptane/n‐butanol/acetonitrile/water (10:19:6:20, v/v/v/v) was employed in the separation and purification of the extracts of P. polyphylla. A total of 7.1 mg polyphyllin VII, 4.3 mg gracillin, 9.2 mg dioscin, and 10.2 mg polyphyllin I were obtained from 1.5 g P. polyphylla in less than 300 min, the purities of which determined by HPLC were 96.7, 97.3, 98.7, and 98.6%, respectively. The identification and characterization of these compounds were performed by LC–ESI‐MS and ¹H NMR spectroscopy. The results demonstrated that the proposed method is feasible, economical and efficient for the extraction, separation and purification of effective compounds from natural products.