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Discovery of multi‐megabase polymorphic inversions by chromosome conformation capture sequencing in large‐genome plant species
- Himmelbach, Axel, Ruban, Alevtina, Walde, Ines, Šimková, Hana, Doležel, Jaroslav, Hastie, Alex, Stein, Nils, Mascher, Martin
- Theplant journal 2018 v.96 no.6 pp. 1309-1316
- barley, chromosome inversions, fluorescence in situ hybridization, genomics, genotype, loci, reproductive isolation
- Chromosomal inversions occur in natural populations of many species, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor‐intensive and lack sensitivity. Here, we report the use of three‐dimensional contact probabilities between genomic loci as assayed by chromosome‐conformation capture sequencing (Hi‐C) to detect multi‐megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi‐C as a generally applicable method for inversion discovery in natural populations.