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Imaging of intracellular-specific microRNA in tumor cells by symmetric exponential amplification-assisted fluorescence in situ hybridization

Author:
Chen, Jun, Yin, Wen, Ma, Yingjun, Yang, Huihui, Zhang, Yanfei, Xu, Mengfei, Zheng, Xuling, Dai, Zong, Zou, Xiaoyong
Source:
Chemical communications 2018 v.54 no.99 pp. 13981-13984
ISSN:
1364-548X
Subject:
chemical compounds, chemical reactions, fluorescence in situ hybridization, image analysis, microRNA, neoplasm cells, risk
Abstract:
We report a symmetric exponential amplification-assisted fluorescence in situ hybridization (SEXPAR-FISH) strategy for imaging intracellular-specific microRNAs (miRNAs) in cells. The strategy eliminates the risk of cell loss and miRNA leakage, and low-abundant intracellular miR-27a in cells can be imaged in only 2 h without compromising sensitivity and specificity.
Agid:
6256822