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Proteoglycan-substrate gel zymography for the detection of chondroitin sulfate-degrading enzymes

Author:
Kobayashi, Takashi, Kakizaki, Ikuko, Nakamura, Toshiya
Source:
Analytical biochemistry 2019 v.568 pp. 51-52
ISSN:
0003-2697
Subject:
cartilage, chondroitin sulfate, enzymes, gels, mammals, metabolism, nose, proteoglycans, salmon
Abstract:
Chondroitin sulfate (CS), a linear polysaccharide, is a major component of the cartilage matrix. Although CS plays various roles in several biological and pathological processes, most details regarding its metabolism are still poorly understood. Some CS-degrading enzymes have been identified in mammals, but their expression patterns and localizations remain unclear. Here we present a simple zymography procedure to detect CS-degrading enzymes using salmon nasal cartilage proteoglycans as substrates. This method should be useful to explore CS-degrading enzymes.
Agid:
6257356