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Generation of Newcastle diease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoprotein gB or gD as dual vaccines

Wei Zhao, Stephen Spatz, Laszlo Zsak, Qingzhong Yu
Vaccine design: Methods and protocols, Volume 2: Vaccines for Veterinary Diseases 2016 v.1404 pp. 89-101
chickens, Newcastle disease, Gallid alphaherpesvirus 1, respiratory tract diseases, live vaccines, virulence, Avian orthoavulavirus 1, viral proteins, viruses, recombinant vaccines, vaccine development, viral vaccines, genetic vectors, glycoproteins
Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens caused by infection of infectious laryngotracheitis virus (ILTV). The current commercial ILT vaccines are either not safe or less effective. Therefore, there is a pressing need to develop safer and more efficacious ILTV vaccines. Newcastle disease (ND), caused by infection with virulent Newcastle disease virus (NDV), is one of the most serious infectious diseases of poultry. The NDV LaSota strain, a naturally occurring low-virulence NDV strain, has been routinely used as a live vaccine throughout the world. This chapter describes the generation of the Newcastle disease virus (NDV) LaSota vaccine strain-based recombinant viruses expressing the glycoprotein B (gB) or D (gD) of ILTV as dual vaccines against ND and ILT using reverse genetics technology.