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A selective and sensitive azido near-infrared fluorescent probe for tris(2-carboxyethyl)phosphine quantitative detection and its application for E. coli determination
- Guo, Hui, Tang, Wei, Duan, Xinrui
- Analytical methods 2018 v.10 no.48 pp. 5823-5826
- Escherichia coli, amino acids, analytical methods, fluorescent dyes, phosphine, thiols
- We developed a sensitive near-infrared fluorescent probe, azido hemicyanine (HC-N₃), for the detection of tris(2-carboxyethyl)phosphine (TCEP) with the lowest LOD (92 nM) so far. HC-N₃ has good selectivity for TCEP among thiols and 20 kinds of natural amino acid. The quantification of E. coli (10¹–10³ cfu mL⁻¹) demonstrated its practicability in complicated biosystems.