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Characterization and functional biology of the soybean aleurone layer

Author:
Schmidt, Monica A., Herman, Eliot M.
Source:
BMC plant biology 2018 v.18 no.1 pp. 354
ISSN:
1471-2229
Subject:
abiotic stress, aleurone layer, biotic stress, endosperm, gene expression, genes, genomics, lipid content, oilseed crops, proteins, rapid methods, seeds, soybeans
Abstract:
BACKGROUND: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean’s genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. RESULTS: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. CONCLUSION: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.
Agid:
6259208