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Overexpression of RcLEC1-B, a HAP3 transcription factor of PLB from Rosa canina, increases the level of endogenous gibberellin and alters the development of cuticle and floral organs in Arabidopsis

Author:
Xu, Kedong, Chang, Yunxia, Wang, Wei, Zhang, Ju, Feng, Bojin, Wang, Cuicui, Liu, Yan, Chen, Qing, Tan, Guangxuan, Li, Chengwei, Zhao, Liangjun
Source:
Gene 2019 v.688 pp. 119-131
ISSN:
0378-1119
Subject:
Arabidopsis, Rosa canina, abscisic acid, amino acids, branches, carpels, flowers, fruits, gene overexpression, genes, gibberellic acid, indole acetic acid, ovules, transcription factors, transgenic plants
Abstract:
The HAP3 subfamily gene RcLEC1-B, was isolated from protocorm-like body (PLB) of Rosa canina, encodes 213 amino acid residues. It was shown that RcLEC1-B was specifically expressed in PLB of R. canina and its subcellular localization is in the nucleus. Overexpression of RcLEC1-B in Arabidopsis resulted in a decrease in endogenous ABA level, an increase in GA, IAA and CTK contents, and an increased number of branches. RcLEC1-B promotes the formation of spontaneous embryoids, suggesting that it may be a homolog of the Arabidopsis LEC1 gene. RcLEC1-B-OE changed the number and morphology of flower organs and resulted in open carpels and exposed ovules, along with a reduced percentage of fertile fruit. This is the first observation that overexpression of a homolog of LEC1 in Arabidopsis can lead to morphological changes in floral organs, cuticle defects, and adhesions between organs; this may result from the increased level of gibberellin in the transgenic plants.
Agid:
6259825