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Genetic analysis of a unique ‘super soft’ kernel texture phenotype in soft white spring wheat

Neeraj Kumar, Jose M. Orenday-Ortiz, Alecia M. Kiszonas, Jeffrey D. Boehm, Craig F. Morris
Journal of cereal science 2019 v.85 pp. 162-167
chromosome mapping, chromosomes, cultivars, flour, genetic analysis, genetic variation, genotyping, inbred lines, linkage groups, parents, phenotype, phenotypic variation, quantitative trait loci, seeds, single nucleotide polymorphism, spring wheat, texture
Kernel texture is a key determinant of flour quality. Herein, a population of 125 recombinant inbred lines (RILs) was developed from a cross between the spring wheat cultivar Alpowa (normal soft) and a closely related uniquely ‘super soft’ line. To study the genetic variation for kernel texture, the RILs and parents were genotyped using high-density single nucleotide polymorphism (SNP) markers. Polymorphic markers (n = 1425) were used for genetic mapping and 372 non-redundant markers were assembled on 21 linkage groups covering 14 chromosomes. Ten QTLs were identified for kernel texture using composite interval mapping (CIM), including four major QTLs on 1BS, 4BS, 5AL and 7AS. Single marker-trait association was also carried out using the same set of markers (1425), and 339 significant markers were identified for kernel texture. Of these 339 markers, the ten best markers, which included the four QTLs above each accounted for 15.0–19.3% phenotypic variation. None of the kernel texture QTL co-located with QTLs for kernel weight or kernel size. This study shows the complex inheritance of this unique super soft kernel trait. Nevertheless, the trait is heritable and could be used to provide bakers a new type of flour.