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Dehydrated and recovered aerobic granules: Identifying acetone-dehydration resistant strains

Lv, Yi, Wan, Chunli, Lee, Duu-Jong, Liu, Xiang, Zhang, Yi, Tay, Joo-Hwa
Bioresource technology reports 2019 v.5 pp. 99-103
Caulobacteraceae, Comamonadaceae, Xanthomonadaceae, acetone, bioactive properties, granules, screening, sludge
The aerobic granules that can be stored at dehydration and rapidly recovered with shape stability and high bioactivities are desired. The strains that can survive under dehydration stress and enrich in the subsequent recovery stage should be incorporated in the seed sludge for granule cultivation. This paper for the first time identified the dehydration resistant strains to family levels by adopting the acetone gradient dehydration-to-stimulation approach. The heterotrophic activity of recovered granules reached a plateau after four dehydration-recovery cycles and the dominating dehydration resistant strains are family Xanthomonadaceae and Comamonadaceae based on high throughput screening. Comparisons between the identified strains after recovery for granule formation, granule stability, freeze/dehydration resistance, and bioactivity functions demonstrate that (Abd El-Rahman et al., 2017) the governing strains for each function are different, and (Cai et al., 2018) only when the seed sludge incorporates the Caulobacteraceae and Xanthomonadaceae the so-yielded granules can be stably stored at dehydrated condition with sufficient recovery capability.