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Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system

Ran Li, Yufeng Zhang, D. Brent Polk, Peggy M. Tomasula, Fang Yan, LinShu Liu
Controlled release journal 2016 v.230 pp. 79-87
Lactobacillus rhamnosus, bioavailability, calcium chloride, colitis, dextran, encapsulation, glucose, homeostasis, human health, humans, hydrocolloids, intestines, mice, microbial physiology, models, pectins, probiotics, proteinases, proteins, sodium sulfate, viability
Probiotics have shown beneficial effects on human health. To increase the efficacy of probiotic applications, we used Lactobacillus rhamnosus GG (LGG) as a probiotic model to investigate approaches to enhance the bioavailability of probiotics. LGG was encapsulated in hydrogel beads containing pectin, a food grade polysaccharide, glucose and calcium chloride. Encapsulated LGG was incubated under conditions that mimicked the physiological condition of the human gastrointestinal tract to evaluate the growth of LGG. Encapsulation increased the acid stability of LGG, protected it from protease digestion, and promoted the production of p40 and p75, two known LGG-derived proteins involved in the beneficial effects of LGG on intestinal homeostasis. To evaluate the effects of encapsulation on the ability of LGG to prevent intestinal injury and colitis, mice were treated with dextran sulphate sodium (DSS) for 4 days with the co-treatment of LGG with and without encapsulation. Administration of encapsulated LGG, but not un-encapsulated LGG, prevented DSS-induced colonic injury and colitis. These data suggest that the encapsulation approach developed in this study improves the survival of LGG and its efficacy for protection of the intestine from inflammatory disorders.