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Structural and functional study for tRNA cleavage by Glycine o-phenanthroline CuII complex, [CuCl(phen)(gly)]∙4H2O

Author:
Jian, Fang fang, Liu, E., Li, Zhuangyu
Source:
Biochemical and biophysical research communications 2019 v.509 no.2 pp. 354-358
ISSN:
0006-291X
Subject:
atomic force microscopy, bacteria, capillary electrophoresis, copper, decontamination, pH, transfer RNA, ultraviolet-visible spectroscopy, viruses
Abstract:
The o-phenanthroline gly Cu(II) complex, [CuCl(phen)(gly)]∙4H2O 1, has been prepared and structurally characterized. The transfer RNA binding and degradation properties of complex 1 have been investigated by UV-vis spectroscopy, cyclic voltammetry (CV), capillary electrophoresis (CE) and atomic force microscopy (AFM) methods. The results showed that 1 can efficiently cleave tRNA in the physiological conditions (pH 7.0, and 37 °C), and has a digestion coefficient nearly up to 100% within 75 h. AFM image for 1/RNA exhibited arrayed tandem repetitions of tRNA segments. This study is targeting the destruction of the high-order structures of genetic biomacromolecules which paves an important way to novel materials for the decontamination of microorganisms (e.g., bacteria and viruses).
Agid:
6263982