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Inhibitory effect of vanillin on the virulence factors and biofilm formation of Hafnia alvei

Li, Tingting, He, Binbin, Mei, Yongchao, Wang, Dangfeng, Sun, Xiaojia, Li, Jianrong
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.102 pp. 223-229
Hafnia alvei, biofilm, computer simulation, fish, genes, hydrogen bonding, lactones, quantitative polymerase chain reaction, quorum sensing, reverse transcriptase polymerase chain reaction, spoilage, vanillin, violacein, virulence
The aim of this study was to evaluate the effect of vanillin as a QS inhibitor at sub-minimal inhibitory concentrations (sub-MICs) against fish spoilage by Hafnia alvei. Biofilm formation, swimming and swarming activity, and production of acyl-homoserine lactones (AHLs) were measured. In addition, analysis of quorum sensing (QS)-related genes by real-time quantitative PCR (RT-qPCR) and molecular docking was performed to better understand the inhibitory mechanism of vanillin. The results indicated that sub-MICs of vanillin induced remarkable reduction in violacein production (59.0%) and biofilm formation (68.5%) along with decreased swimming ability, and reduced expression levels of halI and halR. Moreover, the results of molecular docking revealed that vanillin and C6-HSL could form 2 hydrogen bonds with the LuxR protein (HalR), suggesting a potential competitive tendency between them. Thus, our study provides a basic inhibition mechanism of vanillin and suggests its prospective application as a QS inhibitor.