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Effect of light intensity on the characteristics of algal-bacterial granular sludge and the role of N-acyl-homoserine lactone in the granulation

Author:
Zhang, Bing, Guo, Yuan, Lens, Piet N.L., Zhang, Zhiqiang, Shi, Wenxin, Cui, Fuyi, Tay, Joo Hwa
Source:
The Science of the total environment 2019 v.659 pp. 372-383
ISSN:
0048-9697
Subject:
biofilm, biomass, chemical oxygen demand, lactones, light intensity, microbial communities, municipal wastewater, nitrogen content, particle size distribution, protein synthesis, proteins, quorum sensing, sludge, statistical analysis, tryptophan
Abstract:
The effects of light intensity on the development of algal-bacterial granular sludge (ABGS) were investigated over a period of 12 weeks. The ABGS developed at low light intensity (142 ± 10 μmol m−2·s−1) exhibited excellent settling ability (SVI30 of 30.9 mL/g), COD and TN removal efficiencies (97.6% and 60.4%, respectively). High light intensity (316 ± 12 μmol m−2·s−1) accelerated granular biomass growth (5.3 g/L) and enhanced the TP removal efficiency (83.7%). Extracellular polymeric substance (EPS) analysis revealed that low light intensity induced more large weight distribution protein production (9–12 kDa and 50–150 kDa), predominantly tryptophan and aromatic proteins. Furthermore, N-acyl-homoserine lactones (AHLs) with a side chain ≤ C10 were commonly shared in the ABGS, and the ABGS developed at low light intensity had a higher C6- and 3OC8-HSL content, which effectively promoted the biofilm formation. The add-back studies showed that the AHLs facilitated the regulation of EPS synthesis. Statistical analysis indicated that the AHLs content had a close correlation with the EPS production, the 50th percentile of the particle size distribution and microbial community assembly, suggesting that AHLs-mediated quorum sensing have an important ecological role in EPS expression and algal-bacterial granulation. Overall, this study describes the ABGS development at different light intensities and the mechanisms of ABGS formation treating synthetic domestic wastewater.
Agid:
6264561