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Agaricus bisporus-derived β-glucan prevents obesity through PPAR γ downregulation and autophagy induction in zebrafish fed by chicken egg yolk

Li, Xiumin, Xue, Yu, Pang, Liang, Len, Bo, Lin, Zhichao, Huang, Jiafu, ShangGuan, Zhaoshui, Pan, Yutian
International journal of biological macromolecules 2019 v.125 pp. 820-828
Agaricus bisporus, Danio rerio, adipocytes, adiposity, autophagy, beta-glucans, diet, dose response, egg yolk, larvae, lipid metabolism, lipids, obesity, peroxisome proliferator-activated receptor gamma
β-(1,4)-d-Glucan with (1,2) and (1,6)-linked branches (short for β-glucan), extracted from Agaricus bisporus (Lange) Sing, had significant anti-obesity and lowering-fat effect. FITC-β-glucan was absorbed by adipocytes of zebrafish larvae when stained by Nile Red. β-Glucan decreased the adiposity mass, reduced the expression of ppar g, mtp, L-fabp, ifabp in ISH, which was coincident as the results of RT-PCT. β-Glucan lowered the level of C/EBP α, c SREBP1, LXR α, PPAR γ by WB analysis, which were accompanied by an increase level in LC3 II/LC3 I and a decline level in p62 in dose-dependent manner. This study explored the effect and mechanisms of Agaricus bisporus derived-β-glucan to regulate lipid metabolism and prevent lipid deposits, and provided the experimental data for its use in diet food and food addictive.