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Process optimization, purification and characterization of alkaline stable white laccase from Myrothecium verrucaria ITCC-8447 and its application in delignification of agroresidues

Author:
Agrawal, Komal, Bhardwaj, Nisha, Kumar, Bikash, Chaturvedi, Venkatesh, Verma, Pradeep
Source:
International journal of biological macromolecules 2019 v.125 pp. 1042-1055
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy, Myrothecium verrucaria, X-ray diffraction, absorption, acid insoluble lignin, circular dichroism spectroscopy, delignification, enzyme activity, glucose, ion exchange chromatography, laccase, molecular weight, nutrient content, pH, peptones, polyacrylamide gel electrophoresis, response surface methodology, staining, submerged fermentation, temperature, wheat straw
Abstract:
The white laccase was produced from Myrothecium verrucaria ITCC-8447 under submerged fermentation. The media components were optimized by response surface methodology (CCD-RSM). The nutritional components (glucose and peptone) and physical parameters (pH and temperature) were optimized by response surface methodology for enhanced laccase production by Myrothecium verrucaria ITCC-8447. The enzyme activity under optimum condition exhibited 1.45 fold increases in laccase activity. The white laccase was subjected to ion exchange chromatography with 6 fold purification. The molecular weight of white laccase was ~63–75kDa as estimated by SDS-PAGE followed by the activity staining with ABTS where green bands confirmed the presence of laccase. The enzyme was stable over an alkaline pH range of 7–9 and the temperature range of 30–40°C. The characterization of white laccase was done by CD spectra, UV–visible absorption, FTIR and XRD. The Km and Vmax values of the purified laccase were 2.5mM and1818.2μmol/min/L. The delignification capability of the white laccase was determined by reduction in Kappa number (58.8%) and Klason lignin (64.7%) of wheat straw after 12h of incubation. Further the delignification was confirmed FTIR and XRD.
Agid:
6264758