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Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs

Pérez-Núñez, Daniel, Sunwoo, Sun-Young, Sánchez, Elena G., Haley, Nicholas, García-Belmonte, Raquel, Nogal, Marisa, Morozov, Igor, Madden, Daniel, Gaudreault, Natasha N., Mur, Lina, Shivanna, Vinay, Richt, Juergen A., Revilla, Yolanda
Veterinary immunology and immunopathology 2019 v.208 pp. 34-43
African swine fever, African swine fever virus, DNA, antibodies, cell-mediated immunity, genes, immune response, immunization, interferon-gamma, neutralization, recombinant proteins, swine, vaccines, viral antigens
African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs.Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.