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Cytotoxicity comparison between fine particles emitted from the combustion of municipal solid waste and biomass

Author:
Shang, Yu, Wu, Meiying, Zhou, Jizhi, Zhang, Xing, Zhong, Yufang, An, Jing, Qian, Guangren
Source:
Journal of hazardous materials 2019 v.367 pp. 316-324
ISSN:
0304-3894
Subject:
DNA damage, atomic absorption spectrometry, biomass, combustion, copper, cytotoxicity, endotoxins, heavy metals, human health, interleukin-6, lead, messenger RNA, municipal solid waste, particulates, polychlorinated dibenzodioxins, polychlorinated dibenzofurans, viability, waste incineration, water solubility, zinc
Abstract:
Fine particles (PM2.5) emitted from municipal solid waste incineration (MSWI) contain high amounts of toxic compounds and pose a serious threat to environment and human health. In this study, entire particles as well as extracted water-soluble and -insoluble fractions of PM2.5 collected from MSWI and biomass incineration (BMI) were subjected to physiochemical characterization and cytotoxic tests in A549 and BEAS-2B cells. MSWI PM2.5 had higher contents of heavy metals (including Pb, Zn, and Cu) and dioxins (PCDD/Fs) than did BMI PM2.5. The metals were enriched in the water-insoluble fraction, as measured by inductively coupled plasma-atomic emission spectrometry. BMI PM2.5 had a higher content of endotoxin, which was also enriched in the water-insoluble fraction. MSWI PM2.5 caused more serious cell injuries, as indicated by the lower viability, higher ROS generation, and DNA damage, whereas BMI PM2.5 presented higher pro-inflammatory potential, as indicated by increased mRNA levels of interleukin 6. Normal human BEAS-2B cells were more sensitive than A549 cells in all these tests. Toxic effects caused by MSWI and BMI PM2.5 were mostly attributable to their water-insoluble fractions. Our results indicate different chemical and biological compositions in MSWI and BMI PM2.5 probably dominate in different toxic endpoints in vitro.
Agid:
6265264