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Development of precise GC-EI-MS method to determine the residual fipronil and its metabolites in chicken egg

Author:
Li, Xianjiang, Li, Hongmei, Ma, Wen, Guo, Zhen, Li, Xiuqin, Song, Shanjun, Tang, Hua, Li, Xiaomin, Zhang, Qinghe
Source:
Food chemistry 2019 v.281 pp. 85-90
ISSN:
0308-8146
Subject:
chemical species, detection limit, eggs, fipronil, food safety, gas chromatography-mass spectrometry, insecticides, metabolites, standard deviation, toxicity
Abstract:
Fipronil is a highly toxic insecticide, and fipronil egg scandal greatly threaten people’s health. Herein, a precise and reliable method was developed for the determination of fipronil and three metabolites in chicken egg by GC-EI-MS. Sample pretreatment took about 20 min and baseline separation of four analytes were realized within 12 min by GC–MS. Moreover, the limits of detection were 0.2–0.5 ng g−1 and the limits of quantitation was 0.5–1 ng g−1 with relative standard deviations (RSDs) lower that 3.32%. Satisfactory recoveries were get in the range of 81.16–103.57% for three levels of spiked samples. Finally, the developed method has been applied for the analysis of egg samples from four sources. Results showed that fipronil and fipronil-sulfone were detected in three samples, while fipronil-desulfinyl and fipronil-sulfide were totally absent. The method showed great promising in quantification of fipronil and its metabolites in food safety affairs.
Agid:
6265339