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Transcriptome analysis of the brain of the Chinese mitten crab, Eriocheir sinensis, for neuropeptide abundance profiles during ovarian development
- Liu, Xue, Ma, KeYi, Liu, ZhiQiang, Feng, JianBin, Ye, BaoQing, Qiu, GaoFeng
- Animal reproduction science 2019 v.201 pp. 63-70
- Eriocheir sinensis, allatostatin, brain, cDNA libraries, complementary DNA, databases, diuretic hormone, eclosion hormone, females, messenger RNA, ovarian development, reproduction, sexual maturity, signal transduction, transcription factors, transcriptomics, unigenes
- Neuropeptides, important messenger molecules, regulate various physiological processes, such as growth, development, and reproduction. In the present study, cDNA libraries from brains of E. sinensis were constructed and sequenced using the Illumina technique for transcript analysis and neuropeptides discovery. There were 233,887 transcripts assembled for 194,286 unigenes. According to the annotations of NCBI non-redundant protein (NR) database, 2487 (11.31%) unigenes were annotated successfully. In total, 1273 transcripts were assigned to the “signal transduction mechanisms” category using KOG analysis. The results of KEGG indicate signal transduction and translation pathways were the dominant and enriched signal pathways. Additionally, results indicated C2H2 was the main transcription factor (TF) family. Analysis of the assembled transcripts indicated there were 22 neuropeptide transcripts, such as allatostatin, crustacean female sex hormone, crustacean hyperglycemic hormone, diuretic hormone 31, and eclosion hormone. The detection of these neuropeptides provide for a basic understanding for future study of functions in development, reproduction, and sexual maturation in crustaceans.