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An injectable enzymatically crosslinked tyramine-modified carboxymethyl chitin hydrogel for biomedical applications

Bi, Bo, Liu, Hui, Kang, Wenting, Zhuo, Renxi, Jiang, Xulin
Colloids and surfaces 2019 v.175 pp. 614-624
biocompatibility, biodegradability, biodegradation, chitin, colloids, crosslinking, gelation, hydrogels, hydrogen peroxide, lysozyme, mechanical properties, peroxidase, surgery, thermosensitivity, toxicity testing, tyramine
The in-situ forming injectable hydrogels have received much attention as scaffolds in the biomedical field, providing a minimally invasive surgical procedure to fill the damaged area. In the present work, carboxymethyl chitin (CMCH) synthesized homogenously was further functionalized with tyramine, resulted in a new injectable enzymatically crosslinked in-situ forming hydrogel under physiological conditions. This new tyramine-modified carboxymethyl chitin (CMCH-Tyr) hydrogel showed much better mechanical properties than those of the thermosensitive in-situ forming physical-crosslinking CMCH hydrogel. The CMCH-Tyr hydrogels remained stable under physiological conditions and could be degraded by lysozyme. The gelation time, strength and biodegradation rate of the CMCH-Tyr hydrogels can be adjusted by varying the concentrations of the horseradish peroxidase and H2O2 in the certain range. In vitro cytotoxicity assays and in vivo in-situ injection study showed non-toxicity, favorable gel formation, and good tissue biocompatibility of the enzyme-catalyzed CMCH-Tyr hydrogel. Thus, the biodegradable and biocompatible CMCH-Tyr hydrogels may hold great potential for three dimensional cell culture and tissue engineering.