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A novel method for real-time estimation of insoluble solids and glucose concentrations during enzymatic hydrolysis of biomass

Kadhum, Haider Jawad, Mahapatra, Durga Madhab, Murthy, Ganti S.
Bioresource technology 2019 v.275 pp. 328-337
biomass, corn, cost effectiveness, enzymatic hydrolysis, ethanol, glucose, hydrolysis, lignocellulose, mixing, models, monitoring, slurries, torque
The study describes a novel method using instantaneous mixing torque and rotational speed to estimate insoluble solids and glucose concentrations during enzymatic hydrolysis of biomass. This method is cost-effective for real-time monitoring and control of enzymatic hydrolysis and potentially scalable. The model was developed using biomass slurries at three solids loading (20, 30 and 45%) at various rotational speeds from 50 to 400 rpm. The results showed a significant drop in mixing torque at 12 h with high solids loading. Maximum glucose concentration (205 g/l) during hydrolysis was achieved at 45% solids loading. Insoluble solids and glucose concentration as a function of torque and rotational speeds were modeled using a modified Herschell-Bulkley model. The model describes the experimental observations with high fidelity (R2 = 0.84) and can be used for real time monitoring of many multiphase reaction systems as enzymatic hydrolysis of lignocellulosic biomass and dry grind corn ethanol processes.