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Rapid biosynthesis and characterization of silver nanoparticles from the leaf extract of Tropaeolum majus L. and its enhanced in-vitro antibacterial, antifungal, antioxidant and anticancer properties

Author:
Valsalam, Saritha, Agastian, Paul, Arasu, Mariadhas Valan, Al-Dhabi, Naif Abdullah, Ghilan, Abdul-Kareem Mohammed, Kaviyarasu, K., Ravindran, Balasubramani, Chang, Soon Woong, Arokiyaraj, S.
Source:
Journal of photochemistry and photobiology 2019 v.191 pp. 65-74
ISSN:
1011-1344
Subject:
Fourier transform infrared spectroscopy, Pseudomonas aeruginosa, Tropaeolum majus, X-ray diffraction, antifungal properties, antineoplastic activity, antioxidant activity, bacteria, biosynthesis, cell lines, chemical constituents of plants, crystal structure, doxorubicin, ethanol, gas chromatography-mass spectrometry, infectious diseases, inhibitory concentration 50, ions, leaf extracts, medicinal plants, nanocrystals, nanoparticles, nanosilver, phenylacetic acid, reducing agents, scanning electron microscopy, silver, spectral analysis, therapeutics
Abstract:
Eco-friendly biosynthesis of nanoparticles from medicinal plants as reducing agent has gained importance due to its potential therapeutic uses. In the present study Silver nanoparticles (AgNPs) were eco-friendly synthesized using the leaf extracts of the medicinal plant Tropaeolum majus. The obtained AgNPs were characterized by UV - visible spectrum, FTIR, SEM and XRD which clearly showed the reduction of Ag+ ions to Ag0. In addition, the aqueous and ethanolic extracts were analyzed for phytochemicals and its antioxidant activities. GC–MS spectrum showed the presence of 25 compounds with benzeneacetic acid as the dominant contents. The synthesized AgNPs revealed maximum absorption spectrum at 463 nm and FTIR vibrational peaks at 3357.46, 21,966.52, 2118.42, 1637.27, 658.571 and 411.728 cm−1 respectively. SEM and XRD studies evidenced the nature of nanocrystalline with face centered cubic (fcc) crystal structure. Both AgNPs and plant extracts showed more inhibition activity against Pseudomonas aeroginosa compared to other bacteria with MIC value of 6.25 μg/ml. Antifungal activities was higher for Penicilium notatum with MIC value 31.2 μg/ml. The IC50 values for MCF7 for aqueous extract were found to be 4.68 μg/ml, ethanol extract 7.5 μg/ml, AgNPs 2.49 μg/ml, and doxorubicin 1.4 μg/ml. The IC50 values for VERO cell line for aqueous extract was 8.1 μg/ml, ethanol extract with 6.8 μg/ml, silver nanoparticles 5.3 μg/ml and doxorubicin 2.6 μg/ml respectively. Conclusively, the antibacterial, antifungal, antioxidant and anticancer properties of the synthesized AgNPs from Tropaeolum majus act as major therapeutic drug for microbial infectious disease and other health associated disorders.
Agid:
6266050