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Cultivar Characterization of Tea Seed Oils by Their Active Components and Antioxidant Capacity

Wang, Xiao-qin, Liang, Xing-qiu, Zhao, Jun, Huang, Bing-bing
The journal of the American Oil Chemists' Society 2014 v.91 no.4 pp. 629-639
absorbance, cluster analysis, cultivars, free radical scavengers, genetic relationships, hydroperoxides, oxygen radical absorbance capacity, phenols, principal component analysis, rutin, seed oils, seeds, tea, variance, China
Cultivar characterization of tea seed oils based on their active components and antioxidant capacity was carried out, providing fundamental data for authentication. The seeds were collected from 28 cultivars grown under the same conditions in the region of Anxi county of Fujian province and their oils were analyzed. The results showed that total phenols content (TPC), total flavonoids content, α-tocopherol contents, γ-tocopherol content and δ-tocopherol content were 16.7–529.3 mg GAE/kg of oil, 4.4–208.7 mg rutin/kg of oil, 7.7–347.1 mg/kg of oil, 1.8–106.7 mg/kg of oil, 0.003–35.769 mg/kg of oil, respectively. The antioxidant capacity measured by DPPH radical scavenging activity (DPPH), oxygen radical absorbance capacity (ORAC), absorbance of protein carbonyl (APC) and the absorbance of protein hydroperoxides (APH) were 91.0–2,164.5 μmol/100 g of oil, 251.0–1,209.5 μmol/100 g of oil, 0.014–0.135, 0.034–0.458, respectively. The correlation analysis revealed that the presence of phenolic compounds was significantly correlated with the antioxidant capacity of tea seed oil. Principal Component Analysis revealed the first three components accounted for 81.31 % of the total variance within the data and the main contributor parameters were DPPH, ORAC, APC, APH and TPC. Hierarchical cluster analysis classified the cultivars into three groups, which were in line with the genetic relationship among the cultivars. Our results supplied basic data for the antioxidant mechanism research of tea seed oil and provided necessary information to develop a breeding program directed to tea seed cultivar selections with the high nutraceutical value of tea seed oil.