PubAg

Main content area

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Author:
Barua, Pragya, Lande, Nilesh Vikram, Subba, Pratigya, Gayen, Dipak, Pinto, Sneha, Keshava Prasad, T.S., Chakraborty, Subhra, Chakraborty, Niranjan
Source:
Plant, cell and environment 2019 v.42 no.1 pp. 230-244
ISSN:
0140-7791
Subject:
Cicer arietinum, cell communication, chickpeas, chromosome mapping, circadian clocks, dephosphorylation, food crops, gene expression regulation, genetic engineering, nuclear proteins, phosphoproteins, phosphorylation, phosphotransferases (kinases), protein degradation, proteome, regulatory proteins, seedlings, serine, spliceosomes, stress response, tissues, transcription factors
Abstract:
Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four‐week‐old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72‐ and 144‐hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho‐motif analysis revealed stress‐induced enrichment of proline‐directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time, and circadian clock were observed to undergo dehydration‐induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate‐resilient crops.
Agid:
6266960