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Quantification of Free Ferriprotoporphyrin IX Heme and Hemozoin for Artemisinin Sensitive versus Delayed Clearance Phenotype Plasmodium falciparum Malarial Parasites

Author:
Heller, Laura E., Roepe, Paul D.
Source:
Biochemistry 2018 v.57 no.51 pp. 6927-6934
ISSN:
1520-4995
Subject:
Plasmodium falciparum, artemisinin, biochemistry, cell cycle, developmental stages, heme, mass spectrometry, parasites, phenotype
Abstract:
We use Plasmodium falciparum culture synchronization, optimized heme and hemozoin extraction protocols, and mass spectrometry to quantify the abundance of free ferriprotoporphyrin IX (FPIX) heme and crystallized FPIX (hemozoin; Hz) for various growth stages of intraerythrocytic P. falciparum malarial parasites. Because of altered cell cycle kinetics for delayed clearance phenotype (DCP) parasites relative to that of the control, we test whether FPIX and Hz abundances differ for DCP and control parasites.
Agid:
6267405