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Spontaneous Formation of Gating Lipid Domain in Uniform-Size Peptide Vesicles for Controlled Release

Rahman, Md. Mofizur, Ueda, Motoki, Hirose, Takuji, Ito, Yoshihiro
Journal of the American Chemical Society 2018 v.140 no.51 pp. 17956-17961
biomimetics, drugs, energy transfer, fluorescein, fluorescence, hydrophilicity, mixing, models, permeability, phase transition, phospholipids, polyethylene glycol, polypeptides, temperature
Hybrid assemblies composed of phospholipids and amphiphilic polymers have been investigated previously as a biomimetic model of biological cells. However, these studies focused on the functions of polymers in a sea of membrane lipids. Here, we prepared a highly stable peptide-lipid hybrid vesicle from a combination of an amphiphilic polypeptide and the phospholipid, 1,2-dimyristoyl-sn-glycero-3-phosphocholine, with a mixing molar ratio of 1:1. The phase-separated structure of the hybrid vesicle was demonstrated by fluorescence resonance energy transfer analysis. The lipid domain of the hybrid vesicle had a phase-transition temperature of 38 °C and allowed the permeation of a hydrophilic molecule, fluorescein isothiocyanate-labeled polyethylene glycol (Mw: 2000), above 38 °C. The designed peptide-lipid hybrid vesicle and a “lipidic gate” are a promising tool for smart drug delivery.