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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
- Nurtop, Elif, Villarroel, Paola Mariela Saba, Pastorino, Boris, Ninove, Laetitia, Drexler, Jan Felix, Roca, Yelin, Gake, Bouba, Dubot-Peres, Audrey, Grard, Gilda, Peyrefitte, Christophe, Priet, Stéphane, de Lamballerie, Xavier, Gallian, Pierre
- Virology journal 2018 v.15 no.1 pp. 192
- Zika virus, blood donors, cost effectiveness, dengue, enzyme-linked immunosorbent assay, immunoglobulin G, neutralization tests, screening, serological surveys, seroprevalence, Martinique
- Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.