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Inhibition of selected pathogens inoculated on the surface of catfish fillets by high molecular weight chitosan coating

Karsli, Baris, Caglak, Emre, Li, Dapeng, Rubio, Nancy K., Janes, Marlene, Prinyawiwatkul, Witoon
International journal of food science & technology 2019 v.54 no.1 pp. 25-33
Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, acetic acid, antibacterial properties, aspartic acid, bacteria, catfish, chitosan, coatings, fish fillets, food pathogens, molecular weight, storage temperature, water solubility
Antibacterial activity of high molecular weight water‐soluble (HMWWS) chitosan (800 kDa) was investigated against four Gram‐negative (Escherichia coli, Salmonella typhimurium, Vibrio cholerae and Vibrio parahaemolyticus) and two Gram‐positive (Staphylococcus aureus and Listeria monocytogenes) bacteria. Catfish fillets were surface‐inoculated with these food‐borne pathogens and coated with chitosan dissolved in aspartic acid (AS) or acetic acid (AC) solution at different concentrations (1% or 3%). Samples were stored at 4 °C for 8 days, except for those inoculated with Vibrio species (10 °C for 6 days). Overall, the most effective coating treatment was the 3% HMWWS chitosan in AS solution (800AS3%). Compared with the control, significant (P < 0.05) reductions caused by 800AS3% were observed for all tested pathogens at the end of storage. The growth of V. parahaemolyticus was completely suppressed by 800AS3%. This study demonstrated that HMWWS chitosan in AS solution could be used as an alternative antimicrobial coating for catfish fillets.