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Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production
- Volova, Tatiana, Demidenko, Aleksey, Kiselev, Evgeniy, Baranovskiy, Sergey, Shishatskaya, Ekaterina, Zhila, Natalia
- Applied microbiology and biotechnology 2019 v.103 no.1 pp. 225-237
- Cupriavidus, batch fermentation, biomass, carbon, crystal structure, fermenters, glycerol, molecular weight, polyhydroxyalkanoates, temperature
- The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.