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Immunological activities of polysaccharide extracted from Elaeagnus angustifolia L.

Author:
Sun, Na-xin, Liu, Hui-ping, Liu, Xu-hui, Zhang, Yan, Liu, Xiao-qing, Wang, Shu, Xu, Xiang-xin, Tian, Wen-tan
Source:
CyTA: journal of food 2018 v.16 no.1 pp. 995-1002
ISSN:
1947-6345
Subject:
Elaeagnus angustifolia, blood serum, cyclophosphamide, gel chromatography, immunoglobulin G, interferon-gamma, interleukin-2, mice, molecular weight, phagocytes, phagocytosis, polysaccharides, spleen, thymus gland
Abstract:
The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100 size-exclusion chromatography (G-100). Then the average molecular weight (Mw) of purified polysaccharide was determined by high-performance gel permeation chromatography (HPGPC). One main fraction (EAP-1a) was obtained through the isolation and purification steps. The results showed that EAP-1a had strong immunological activities. EAP-1a could significantly increase spleen and thymus index, enhance the NK cell activity, stimulate the phagocytosis of peritoneal phagocyte and increase the level of IL-2, IFN-γ and IgG in serum. Thus, EAP-1a isolated from Elaeagnus angustifolia L. could enhance the immunological activities of immunosuppressed mice induced by cyclophosphamide (CY).
Agid:
6268547