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Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis

Kushkevych, Ivan, Dordević, Dani, Kollár, Peter
Open life sciences 2019 v.13 no.1 pp. 481-488
Desulfovibrio, acetates, biomass production, colitis, feces, hydrogen sulfide, inflammation, intestines, lactic acid, metabolism, oxidation, patients, principal component analysis, regression analysis, sulfate-reducing bacteria, sulfates
Intestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction in Desulfovibrio species that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations, p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R²) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H₂S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.