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Single-site labeling of histidine in proteins, on-demand reversibility, and traceless metal-free protein purification
- Joshi, Pralhad Namdev, Rai, Vishal
- Chemical communications 2019 v.55 no.8 pp. 1100-1103
- Lewis bases, chemical bonding, chemical reactions, histidine, proteins
- A precision methodology distinguishes one His from all the nucleophilic residues and its multiple copies. An easy-to-operate C–N bond formation labels diverse proteins without adversely affecting their structure and function. The late-stage transformation allows installation of distinct probes. The chemically triggered reversibility enables traceless metal-free purification of proteins with a His-tag.