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Genetically encoded RNA-based sensors for intracellular imaging of silver ions
- YuThese two authors contribute equally to this work., Qikun, Shi, Jing, Mudiyanselage, Aruni P. K. K. Karunanayake, Wu, Rigumula, Zhao, Bin, Zhou, Ming, You, Mingxu
- Chemical communications 2019 v.55 no.5 pp. 707-710
- RNA, antibacterial properties, bacteria, broccoli, chemical reactions, disinfection, image analysis, ions, oligonucleotides, silver
- Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag⁺) is critical in these antibacterial effects. The direct cellular measurement of Ag⁺ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag⁺ in live bacterial cells. The sensor is designed by introducing a cytosine–Ag⁺–cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag⁺ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag⁺.