PubAg

Main content area

Genetically encoded RNA-based sensors for intracellular imaging of silver ions

Author:
YuThese two authors contribute equally to this work., Qikun, Shi, Jing, Mudiyanselage, Aruni P. K. K. Karunanayake, Wu, Rigumula, Zhao, Bin, Zhou, Ming, You, Mingxu
Source:
Chemical communications 2019 v.55 no.5 pp. 707-710
ISSN:
1364-548X
Subject:
RNA, antibacterial properties, bacteria, broccoli, chemical reactions, disinfection, image analysis, ions, oligonucleotides, silver
Abstract:
Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag⁺) is critical in these antibacterial effects. The direct cellular measurement of Ag⁺ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag⁺ in live bacterial cells. The sensor is designed by introducing a cytosine–Ag⁺–cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag⁺ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag⁺.
Agid:
6269214