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Ratiometric DNA sensing with a host–guest FRET pair

Author:
Zhang, Shuai, Assaf, Khaleel I., Huang, Chusen, Hennig, Andreas, Nau, Werner M.
Source:
Chemical communications 2019 v.55 no.5 pp. 671-674
ISSN:
1364-548X
Subject:
4',6-diamidino-2-phenylindole, DNA, chemical reactions, macrocyclic compounds
Abstract:
A supramolecular host–guest FRET pair based on a carboxyfluorescein-labelled cucurbit[7]uril (CB7-CF, as acceptor) and the fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI, as donor) is developed for sensing of DNA. In comparison to the commercial DNA staining dye SYBR Green I, the new chemosensing ensemble offers dual-emission signals, which allows a linear ratiometric response over a wide concentration range.
Agid:
6269249