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Photo-regulatable DNA isothermal amplification by template-mediated ligation
- Cheng, Bohao, Kashida, Hiromu, Shimada, Naohiko, Maruyama, Atsushi, Asanuma, Hiroyuki
- Chemical communications 2019 v.55 no.8 pp. 1080-1083
- DNA, chemical reactions, irradiation, lysine, oligonucleotides, polymers
- By combining azobenzene-tethered oligonucleotides as modulators and poly(l-lysine)-graft-dextran (PLL-g-Dex), a chaperone polymer, to facilitate strand displacement, we successfully developed a photo-regulatable DNA isothermal amplification method. By alternating UV and visible irradiation, linear amplification was achieved. The method enables photo-regulatability and mismatch discrimination in linear amplification of the DNA target.